Partition of α-lactoalbumin and β-lactoglobulin by cloud point extraction
نویسندگان
چکیده
منابع مشابه
Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized Microarray
BACKGROUND α-Lactalbumin (a-LA), β-lactoglobulin (β-LG) and lactoferrin (LF) are of high nutritional value which have made ingredients of choice in the formulation of modern foods and beverages. There remains an urgent need to develop novel biosensing methods for quantification featuring reduced cost, improved sensitivity, selectivity and more rapid response, especially for simultaneous detecti...
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Cloud point extraction (CPE) as a rapid, simple and efficient method coupled with high performance liquid chromatography (HPLC) was used for sample preparation and subsequent determination of carbazole, trinitrocarbazole (TrNC) and tetra nitro carbazole (TNC) in water samples. Some effective parameters on extraction, such as volume of Triton X-100, extraction time, extraction temperature, ionic...
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The aim of this work was to develop a selective cloud point extraction method for the separation and preconcentration of copper(II) prior to spectrophotometric determination. For this purpose dithizone was used as a complexing agent and the experimental solution was acidified with sulfuric acid. Triton X-114 was used as a surfactant and after phase separation, based on the cloud point of th...
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Pb and Cd in water samples were determined by cloud point extraction (CPE) with sodiumdiethyldithiocarbamate (DDTC) as the chelating agent and octylphenoxypolyethanl (Triton X-114) asthe surfactant prior to flame atomic absorption spectrometry (FAAS) determination. The main factorsof affecting the extraction and detection processes were optimized. The preconcentration of 10 mL ofstandard soluti...
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In this work, we describe a simple, green and general procedure for the purification and fractionation of carbon dots (CDs). CDs coated with oxygen-containing functional groups were synthesized by thermal pyrolysis of citric acid. The product of the reaction was first Purified and then fractionated into two distinct kinds of CDs (f4 and f1) using pH-controlled cloud point extraction (CPE) techn...
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ژورنال
عنوان ژورنال: Journal of Chromatography B
سال: 2008
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2008.03.028